課程大綱

課程資訊

課程名稱	生物技術核心實驗 Biotechnology Core Techniques
開課學期	102-2
授課對象	生物技術學程
授課教師	杜宜殷
課號	Prog5074
課程識別碼	P05 U2020
班次	01
學分	4
全/半年	半年
必/選修	必修
上課時間	星期二2,3,4,@,5,6,7,8(9:10~16:20)
上課地點	生技館213
備註	需於電腦初選期間(1/13~1/23)至生技中心報名，需繳學分費。週二第@節請勿排他課。與陳仁治、張世宗合開總人數上限：40人
Ceiba 課程網頁	http://ceiba.ntu.edu.tw/1022Prog5074_01
課程簡介影片
核心能力關聯	本課程尚未建立核心能力關連
課程大綱
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課程概述	Students will be asked to choose a topic on horticulture, collect, read, and organize literatures, write up abstract, oral present with media aid, control report time, ask and answer questions, write up report, and service.
課程目標	This course will train senior students to know the research trend of the world. Students are asked to collect and organize references, dig out questions, innovate ideas, write a report, oral present, answer questions properly, and obey to the rules. These abilities are required for both advanced study and job working.
課程要求	Present an oral and written report on horticulture related topics and discuss in classes.
預期每週課後學習時數
Office Hours
指定閱讀	待補
參考書目	Journals and books related on horticulture, plant science, food processing, and biotechnology.
評量方式 (僅供參考)

課程進度

週次	日期	單元主題
第2週	2/25	1. Introduction 2. Cloning tools
第3週	3/04	Cloning strategies
第4週	3/11	Isolation and quantitative analysis of DNA
第5週	3/18	Transformation and identification of transformants
第6週	3/25	Protein expression systems
第7週	4/01	春假
第8週	4/08	Detection of specific nucleic acid sequences by hybridization
第9週	4/15	1. RNA in general 2. RNA preparation
第10週	4/22	Denaturing gel electrophoresis
第11週	4/29	1.Probe preparation 2. Northern hybridization analysis
第12週	5/06	Analysis of RNA by primer extension, S1 analysis, RNase protection and nuclear run-off
第13週	5/13	Protein in general Protein extraction Gel filtration chromatography
第14週	5/20	Ion exchange chromatography Affinity chromatography
第15週	5/27	Protein assay Polyacrylamide electrophoresis protein transfer
第16週	6/03	Immunological tools
第17週	6/10	Protein Examination